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Series GSE89654 Query DataSets for GSE89654
Status Public on Aug 01, 2017
Title Expression data from Treg cells expressing mutant FoxP3
Organism Mus musculus
Experiment type Expression profiling by array
Summary FoxP3 is a central regulator of immunological tolerance, controlling the development and function of regulatory T (Treg) cells. To dissect the complex processes orchestrated by FoxP3, we investigated impacts of three autoimmune disease-associated missense FoxP3 mutations (i.e., I363V, A384T, R397W) through knock-in mutagenesis in mice.
We investigated the impacts of these mutations on Treg cell transcriptome by microarray analysis.
Overall design CD4(+)hCD2(+) WT or mutant Treg cells were sorted from FoxP3WT or mutant:hCD2/WT:GFP heterozygous mice. CD4(+)GFP(+) WT Treg cells were also sorted from FoxP3WT or A384T:hCD2/WT:GFP mice. CD4(+)hCD2(-)GFP(-) Tconv cells were also obtained from FoxP3WT:hCD2/WT:GFP mice. All cell populations analyzed were generated in duplicates or triplicates.
Contributor(s) Tachibana M, Hori S
Citation(s) 28778586
Submission date Nov 08, 2016
Last update date Feb 11, 2019
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM2386159 Tconv_1
GSM2386160 Tconv_2
GSM2386161 WT Treg (GFP+ from WT:hCD2/WT:GFP mice)_1
This SubSeries is part of SuperSeries:
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells
BioProject PRJNA352831

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89654_RAW.tar 70.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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