|
| Status |
Public on Apr 15, 2008 |
| Title |
RM5E RM11-1a 1% ethanol rep 1 vs mixed reference |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
RM5E RM11-1a 1% ethanol rep 1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain Name : RM11-1a
|
| Biomaterial provider |
Erin Smith, Kruglyak Lab, Princeton University
|
| Growth protocol |
media : "ethanol carbon source: 6.7g/L YNB (Sigma Y0626), 10mL/L ethanol, 100 mg/L leucine, 100mg/L lysine, 20mg/L uracil"
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method : "Hot acid/phenol, Qiagen Rneasy Kit"
|
| Label |
Cy3
|
| Label protocol |
Type of label : Cy3
Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 4, January 2006, Agilent Technologies, Publication 5185-58
|
| |
|
| Channel 2 |
| Source name |
BYg+RMg+BYE+RME
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain Name : common reference: mixture of BY4716 and RM11-1a RNA from glucose and ethanol conditions
|
| Biomaterial provider |
Erin Smith, Kruglyak Lab, Princeton University
|
| Growth protocol |
media : common reference: mixture of BY4716 and RM11-1a RNA from glucose and ethanol conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method : "Hot acid/phenol, Qiagen Rneasy Kit"
|
| Label |
Cy5
|
| Label protocol |
Type of label : Cy5
Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 4, January 2006, Agilent Technologies, Publication 5185-58
|
| |
|
| |
| Hybridization protocol |
Hybridization date : 2/27/07
Two-Color Microarray-Based Gene Expression Analysis v.5.5, Agilent Technologies, Publication G4140-90050 V.5.5
|
| Scan protocol |
Pixel Size : 10
Scan Date : 2007-02-27
Scan Time : 14:06:45
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
|
| Description |
RM5E RM11-1a 1% ethanol rep 1 vs mixed reference
|
| Data processing |
Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.1.3.1
Datafile type : Agilent result file
|
| |
|
| Submission date |
Oct 17, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Erin N Smith |
| E-mail(s) |
ensmith@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Ecology and Evolutionary Biology
|
| Lab |
Kruglyak
|
| Street address |
110 Carl Icahn Labs
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
| |
|
| Platform ID |
GPL2883 |
| Series (1) |
| GSE9376 |
Gene-environment interaction in yeast gene expression |
|
