|
| Status |
Public on Nov 01, 2007 |
| Title |
3-day-old TJ1090 worms with oxidative stress (O1) |
| Sample type |
RNA |
| |
|
| Source name |
control worms with oxidative stress (O1)
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
Strain: TJ1090
Gender: hermaphrodite
Age: 3 days old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Worms were harvested by washing off plates with S-basal and quick frozen in liquid nitrogen. Total RNA was prepared using TRIZOL reagent and standard protocols and cleaned up on Qiagen RNeasy columns.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared by the RT-IVT method from 10 microg total RNA using the BioArray-High-Yield-RNA-Transcript-Labeling-Kit (Enzo, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix GeneChip C. elegans Genome Array according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 7G high resolution laser scanner.
|
| Description |
Gene chip hybridization and scanning were done at Genome Explorations (Memphis, MN) and the University of Colorado at Boulder Gene Chip Core Facility (Boulder, CO).
|
| Data processing |
The data were background corrected and summarized with MAS 5.0 using Affymetrix GCOS v1.4 software (Affymetrix, Santa Clara, CA). The normalization target (TGT) was set to 250.
|
| |
|
| Submission date |
Oct 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sang-Kyu Park |
| E-mail(s) |
sangkyu@colorado.edu
|
| Phone |
303-492-5159
|
| Fax |
303-492-8063
|
| Organization name |
University of Colorado at Boulder
|
| Department |
IBG
|
| Street address |
1480 30th St.
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80303 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE9301 |
SKN-1-dependent oxidative stress response in C. elegans |
|
