|
| Status |
Public on Sep 19, 2007 |
| Title |
control (unstimulated) cells, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human THP-1 macrophages, unstimulated
|
| Organism |
Homo sapiens |
| Characteristics |
Monocyte cell line, can be differentiated to macrophage, ATCC no. TIB-202
|
| Treatment protocol |
Standard media was replaced with serum-free media containing 2% BSA, lipoproteins (in PBS) or the equivalent volume of PBS (controls) were added, incubaton was carried out for 24h, cells were then washed twice in PBS, scraped and stored at -80deg
|
| Growth protocol |
Standard THP-1 cell media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
By Rneasy system (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA prepared as standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol EukGE-WS2v5_450
|
| Scan protocol |
Arrays were scanned using an Affymetrix GeneChip® Scanner 3000
|
| Description |
Gene expression data
|
| Data processing |
dChip (build April 15, 2005) was used for normalization and modeling using the PM-only model, Control rep1 as baseline array
|
| |
|
| Submission date |
Sep 19, 2007 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Silvio Zaina |
| Organization name |
Rigshospitalet
|
| Department |
Clinical Biochemistry
|
| Street address |
Blegdamsvej 9
|
| City |
COPENHAGEN |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9101 |
Expression data in native lipoprotein-stimulated human THP-1 macrophages |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
| Reanalyzed by |
GSE134312 |
