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| Status |
Public on Sep 19, 2007 |
| Title |
Expression data in native lipoprotein-stimulated human THP-1 macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We previously found that a native lipoprotein mix with a high VLDL+LDL/HDL ratio causes a global de novoDNA methylation in THP-1 macrophages. In the present experiment we assessed the consequences of global lipoprotein-induced de novo DNA methylation on global gene expression in the same cells. Moreover, we sought to use gene expression array data to measure RNA expression levels for candidate factors mediating the epigenetic effects of lipoproteins.
Keywords: comparison treated vs. control
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| Overall design |
Human native VLDL, LDL and HDL lipoproteins were isolated from buffy coats, fractionated by ultracentrifugation, stored at -80deg., desalted to PBS before usage and kept at 4deg. for a maximum of 7d. THP-1 monocytes were differentiated to macrophages, stimulated for 24h with a mix of 68.8mg/ml VLDL, 32.1mg/ml LDL, 91.1mg/ml HDL or unstimulated (control), in serum-free medium with 2% BSA. Three independently isolated lipoprotein samples were used in triplicate.
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| Contributor(s) |
Schjering C, Smed S |
| Citation(s) |
22118513 |
| Submission date |
Sep 19, 2007 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Silvio Zaina |
| Organization name |
Rigshospitalet
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| Department |
Clinical Biochemistry
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| Street address |
Blegdamsvej 9
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| City |
COPENHAGEN |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM230349 |
control (unstimulated) cells, biological rep1 |
| GSM230350 |
control (unstimulated) cells, biological rep2 |
| GSM230351 |
control (unstimulated) cells, biological rep3 |
| GSM230352 |
cells stimulated with lipoprotein prep 1, biological rep1 |
| GSM230353 |
cells stimulated with lipoprotein prep 1, biological rep2 |
| GSM230354 |
cells stimulated with lipoprotein prep 1, biological rep3 |
| GSM230355 |
cells stimulated with lipoprotein prep 2, biological rep1 |
| GSM230356 |
cells stimulated with lipoprotein prep 2, biological rep2 |
| GSM230357 |
cells stimulated with lipoprotein prep 2, biological rep3 |
| GSM230358 |
cells stimulated with lipoprotein prep 3, biological rep1 |
| GSM230359 |
cells stimulated with lipoprotein prep 3, biological rep2 |
| GSM230360 |
cells stimulated with lipoprotein prep 3, biological rep3 |
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| Relations |
| BioProject |
PRJNA102627 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9101_RAW.tar |
48.5 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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