|
| Status |
Public on Oct 11, 2016 |
| Title |
D2_8w |
| Sample type |
SRA |
| |
|
| Source name |
Skin
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 x 129
tissue: Skin
age: 8 weeks
genotype: Stat3f/f K5cre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Skin were removed, treated with RNALater, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Read alignment: TopHat2 v2.0.8 with default settings
expression level: Cufflinks v2.1.1 with default settings
Genome_build: mm9
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Aug 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kosuke Miyauchi |
| E-mail(s) |
kosuke.miyauchi@riken.jp
|
| Organization name |
RIKEN Center for Integrative Medical Sciences
|
| Street address |
1-7-22 Suehiro-sho
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE86071 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Stat3f/f K5cre Skin Transcriptomes |
|
| Relations |
| BioSample |
SAMN05660802 |
| SRA |
SRX2053685 |
