|
| Status |
Public on Dec 01, 2017 |
| Title |
G25-3 |
| Sample type |
SRA |
| |
|
| Source name |
cardiac progenitor cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ES-derived cardiac progenitor cells
treatment: Glucose 25 mM
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
TRIZOL (TheroFisher) and RNeasy kit (QIAGEN)
Libraries for RNA-Seq in Figure 1G and H were prepared with KAPA Stranded RNA-Seq Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
replicate C
|
| Data processing |
Read-Alignment: Short-read sequences generated from Illumina Sequencer were aligned to the UCSC human reference genome hg19 downloaded from support.Illumina.com (http://support.illumina.com/sequencing/sequencing_software/igenome.html) using TopHat from the Tuxedo Tools.
Abundance measurements: Counts/gene expression matrices were generated using HTSeq which quantified the reads per transcript
Normalization of abundance measurements: The expression matrices were log transformed and normalized using the rlog function in Deseq2 package.
Genome_build: hg19
Supplementary_files_format_and_content: tab delimited text files include expression values for annotated features in each sample
|
| |
|
| Submission date |
Jul 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
matteo pellegrini |
| E-mail(s) |
matteop@mcdb.ucla.edu
|
| Phone |
310 825-0012
|
| Organization name |
UCLA
|
| Street address |
610 charles young drive east
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE84814 |
Glucose inhibits cardiac maturation through nucleotide biosynthesis |
|
| Relations |
| BioSample |
SAMN05439279 |
| SRA |
SRX1975937 |
