|
Status |
Public on Dec 01, 2017 |
Title |
G-2 |
Sample type |
SRA |
|
|
Source name |
cardiac progenitor cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: ES-derived cardiac progenitor cells treatment: Glucose 0 mM for 5 days and back to Glucose 25 mM for 5 days
|
Extracted molecule |
polyA RNA |
Extraction protocol |
TRIZOL (TheroFisher) and RNeasy kit (QIAGEN) Libraries for RNA-Seq in Figure 1G and H were prepared with KAPA Stranded RNA-Seq Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
replicate B
|
Data processing |
Read-Alignment: Short-read sequences generated from Illumina Sequencer were aligned to the UCSC human reference genome hg19 downloaded from support.Illumina.com (http://support.illumina.com/sequencing/sequencing_software/igenome.html) using TopHat from the Tuxedo Tools. Abundance measurements: Counts/gene expression matrices were generated using HTSeq which quantified the reads per transcript Normalization of abundance measurements: The expression matrices were log transformed and normalized using the rlog function in Deseq2 package. Genome_build: hg19 Supplementary_files_format_and_content: tab delimited text files include expression values for annotated features in each sample
|
|
|
Submission date |
Jul 25, 2016 |
Last update date |
May 15, 2019 |
Contact name |
matteo pellegrini |
E-mail(s) |
matteop@mcdb.ucla.edu
|
Phone |
310 825-0012
|
Organization name |
UCLA
|
Street address |
610 charles young drive east
|
City |
los angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL21290 |
Series (1) |
GSE84814 |
Glucose inhibits cardiac maturation through nucleotide biosynthesis |
|
Relations |
BioSample |
SAMN05439266 |
SRA |
SRX1975933 |