|
| Status |
Public on Dec 30, 2018 |
| Title |
Blood_CKDu Stage 5_rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral whole blood, Stage 5 CKDu, male pool
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
diagnosis: CKDu
Stage: Stage 5
gender: Male
occupation: Farming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from peripheral whole blood using Qiazol lysis reagent (Qiagen, Netherlands) and column purified using PAXgene spin columns (Qiagen, Netherlands)
|
| Label |
Biotinylated cRNA
|
| Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX)
|
| |
|
| Hybridization protocol |
Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina Expression Beadchip according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner
|
| Description |
U5_2
|
| Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2011.1 (Gene Expression Module v1.9.0).
|
| |
|
| Submission date |
Jul 22, 2016 |
| Last update date |
Dec 30, 2018 |
| Contact name |
Dhammika Magana-Arachchi |
| E-mail(s) |
dmaganaarachchi@gmail.com
|
| Organization name |
National Institute of Fundamental Studies
|
| Department |
Cell Biology
|
| Lab |
Cell Biology
|
| Street address |
Hantana Road
|
| City |
Kandy |
| State/province |
Central |
| ZIP/Postal code |
20000 |
| Country |
Sri Lanka |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE62792 |
Whole genome expression in peripheral whole blood of chronic kidney disease patients having uncertain etiology (CKDu) compared to healthy controls |
|
