|
| Status |
Public on Jun 09, 2017 |
| Title |
Tumor-0Gy-24hrs-2 |
| Sample type |
RNA |
| |
|
| Source name |
TSA mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted after homogenization of the sample using miRNeasy Mini kit (Qiagen) according to the manufacturer's protocol.
|
| Label |
Alexa-555
|
| Label protocol |
Total RNA was reverse transcribed after priming with a DNA oligonucleotide containing the T7 RNA polymerase promoter 5’ to a d(T)24 sequence. After second-strand cDNA synthesis and purification of double-stranded cDNA, in vitro transcription was performed using T7 RNA polymerase. The quantity and quality of the cRNA was assayed by spectrophotometry and on the Agilent Bioanalyzer as indicated for Total RNA analysis.
|
| |
|
| Hybridization protocol |
Purified cRNA was fragmented to uniform size and applied to Agilent Mouse Gene Expression 4x44 v2 Microarray (Agilent Technologies, design ID 026655) in hybridization buffer. Arrays were hybridized at 37° C for 18 hrs. in a rotating incubator.
|
| Scan protocol |
Arrays were scanned on an Agilent G2505B Microarray Scanner
|
| Data processing |
All arrays were processed with Agilent Feature Extraction software and data was analyzed with GeneSpring GX software (Agilent Technologies).
|
| |
|
| Submission date |
Jun 30, 2016 |
| Last update date |
Jun 10, 2017 |
| Contact name |
Sandra Demaria |
| E-mail(s) |
szd3005@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Department |
Radiation Oncology
|
| Lab |
Demaria Lab
|
| Street address |
1300 York avenue, Room A-279
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE83915 |
Comparison of gene expression changes in TSA tumors following different radiation regimens. |
|
