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Series GSE83915 Query DataSets for GSE83915
Status Public on Jun 09, 2017
Title Comparison of gene expression changes in TSA tumors following different radiation regimens.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Radiotherapy is under investigation in the clinic for its ability to induce an in situ vaccine and enhance responses to immune checkpoint inhibitors and other immunotherapies. A variety of radiation doses and delivery schedules have been used to induce anti-tumor T cells in preclinical studies. However, the mechanisms bu which radiation induces anti-tumor T cells against poorly immunogenic tumors remain incompletely understood. In this study, we investigated changes in gene expression in mouse mammary carcinoma TSA tumors after a single dose (SD) of 20 Gy and a multi-fraction (MF) radiation regimen of 3x8Gy. To this end, TSA cells were injected into syngeneic BALB/c mice. When tumors became palpable, they were treated with local radiation therapy (RT) and harvested 4 or 24 hours later for gene expression analysis. The genome-wide microarray analysis showed key differences between SD and MF RT that may explain the differential ability of these two RT regimens to synergize with immune checkpoint inhibitors (Dewan et al., Clin Cancer Res 2009).
Overall design To better understand why multi-fractionated (MF - 3x8Gy) radiation regimen is better at synergizing with anti-CTLA4 compared to a single dose (SD) of 20Gy, we harvested TSA tumors 4 and 24 hours after completion of RT. Three tumors from each group were then subjected to RNA extraction and hybridization on Agilent array.
Contributor(s) Demaria S, Formenti SC, Coleman CN, Arankalayil MJ, Vanpouille-Box C
Citation(s) 28598415
Submission date Jun 30, 2016
Last update date Nov 01, 2017
Contact name Sandra Demaria
Organization name Weill Cornell Medicine
Department Radiation Oncology
Lab Demaria Lab
Street address 1300 York avenue, Room A-279
City New York
State/province New York
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (18)
GSM2221400 Tumor-0Gy-4hrs-1
GSM2221401 Tumor-0Gy-4hrs-2
GSM2221402 Tumor-0Gy-4hrs-3
BioProject PRJNA327399

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83915_RAW.tar 161.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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