|
| Status |
Public on Jun 13, 2017 |
| Title |
IFC (degradome) |
| Sample type |
SRA |
| |
|
| Source name |
tissue: ovary
|
| Organism |
Zea mays |
| Characteristics |
developmental state: before pollination
tissue: ovary
phenotype: incompletely fused carpels (IFC)
|
| Treatment protocol |
Under non-stress conditions.
|
| Growth protocol |
Zea mays L. (maize) inbred Yu-A474 plants were grown at a research farm of Henan Agricultural University, Henan Province, China (34º53´N, 113 º35´E, 94 m altitude) under non-stress conditions during June-October 2014.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Library strategy: degradome-Seq
A proprietary pipeline script, ACGT101-miR v4.2 (LC Sciences), was used for sequencing data analysis (Li et al. 2010; Wei et al. 2011). A Public software package, CleaveLand3.0.1 was used for analyzing Degradome sequencing data.
|
| |
|
| Submission date |
May 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
hongping li |
| E-mail(s) |
lmhlhp021506@163.com
|
| Organization name |
Henan Agricultural University
|
| Street address |
63
|
| City |
zhengzhou |
| ZIP/Postal code |
450002 |
| Country |
China |
| |
|
| Platform ID |
GPL17628 |
| Series (1) |
| GSE80998 |
Incompletely fused carpels ovary development revealed by miRNAs and target genes analysis in maize. (smallRNA-Seq and degradome-Seq) |
|
| Relations |
| BioSample |
SAMN04931541 |
| SRA |
SRX1738304 |
