|
| Status |
Public on Nov 03, 2017 |
| Title |
ful-1_DEX_2 |
| Sample type |
RNA |
| |
|
| Source name |
dipped in 10 uM DEX, harvested after 8 hrs
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col/Ler F2
genotype: ful-1
developmental stage: Flower stage 12-16 (Smyth et al., 1990)
tissue: pistils/siliques
|
| Treatment protocol |
Inflorescences were dipped into 10 µM Dexamethasone (DEX) solution (10 µM DEX, 0.5 MS, 0.02% Silwet) or Mock solution (0.5 MS, 0.02% Silwet), and pistils/siliques were harvested after 8 hours.
|
| Growth protocol |
Plants were grown on soil in a growth chamber at 22 0C and 16/8 h day/night
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and treated with DNase.
|
| Label |
biotin
|
| Label protocol |
According to Rehrauer et al. Plant Physiology (2010). DOI 10.1104/pp.109.150185
|
| |
|
| Hybridization protocol |
According to Rehrauer et al. Plant Physiology (2010). DOI 10.1104/pp.109.150185
|
| Scan protocol |
According to Rehrauer et al. Plant Physiology (2010). DOI 10.1104/pp.109.150185
|
| Data processing |
RMA was used to normalize the data; Limma package was used to detect differential expression. RMA values are available on associated GSE entry.
|
| |
|
| Submission date |
Mar 23, 2016 |
| Last update date |
Nov 03, 2017 |
| Contact name |
Marian Bemer |
| E-mail(s) |
marian.bemer@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Molecular Biology
|
| Street address |
Droevendaalsesteeg 1
|
| City |
Wageningen |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL14926 |
| Series (2) |
| GSE79553 |
Expression profile of Arabidopsis pistil/silique upon induction of FRUITFULL |
| GSE79555 |
Expression profiling and identification of targets of FRUITFULL in the Arabidopsis pistil/silique |
|
