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| Status |
Public on Nov 03, 2017 |
| Title |
Expression profile of Arabidopsis pistil/silique upon induction of FRUITFULL |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To determine the genes that are regulated by FRUITFULL (FUL) in the pistil/silique, we identied differentially expressed genes after induction of FRUITFULL in ful-1 FUL-GR lines using the AGRONOMICS1 tiling array. The inflorescences of flowering plants were dipped in 10 uM DEX solution or in Mock Solution, and the flowers/siliques from stages 12-16 were harvested after 8 hours. RNA was extracted and DNase treated, and subsequent steps and hybridization to the AGRONOMICS Tiling array were performed by the Functional Genomics Center Zurich.
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| Overall design |
We generated several control samples to be able to identify genes that were differentially regulated by the DEX alone treatment instead of by the induction of FUL. A considerable number of genes was found to respond to DEX (samples ful-1 DEX vs ful-1 Mock). To identify the genes that were actually differentially upregulated because of FUL, we compared the samples ful-1_FUL-GR_DEX and ful-1_FUL_GR_Mock). All samples were performed in duplicate.
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| Contributor(s) |
Bemer M, Muino J, Angenent G |
| Citation(s) |
28586421 |
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| Submission date |
Mar 23, 2016 |
| Last update date |
Nov 03, 2017 |
| Contact name |
Marian Bemer |
| E-mail(s) |
marian.bemer@wur.nl
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| Organization name |
Wageningen University
|
| Department |
Molecular Biology
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| Street address |
Droevendaalsesteeg 1
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| City |
Wageningen |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL14926 |
Affymetrix AGRONOMICS Tiling Array [CDF: agronomics1_TAIR9_gene] |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE79555 |
Expression profiling and identification of targets of FRUITFULL in the Arabidopsis pistil/silique |
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| Relations |
| BioProject |
PRJNA316153 |
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