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Sample GSM2044364 Query DataSets for GSM2044364
Status Public on Aug 01, 2016
Title PBMC_control_rep2
Sample type RNA
Source name PBMC, healthy infant, replicate 2
Organism Homo sapiens
Characteristics diagnosis: healthy
tissue: whole blood
cell type: PBMC
Growth protocol PBMC was isolated by density gradient centrifugation on Percoll (d = 1.077) .
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions and purified by RNeasy MinElute Cleanup kit.
Label Cy3
Label protocol Complementary DNA amplification was carried out from 35 ng of total RNA using an Ovation PicoSL WTA System V2. After amplification, cDNA samples were labeled with Cy3 using Klenow polymerase.
Hybridization protocol Slide was hybridized with 1.65 μg Cy3-labeled cDNA using a Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, USA) in a hybridization oven.
Scan protocol After 17 h of hybridization at 65 °C, the slide was washed with a Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions. Slides were scanned after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides.
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The intensity signals was imported to CLC Main workbench 6.0 and normalized by the quartile normalization method and log2 transformed.
Submission date Jan 21, 2016
Last update date Aug 01, 2016
Contact name Ryo Inoue
Organization name Kyoto Prefectural University
Street address 1-5, Hangi-cho, shimogamo
City Kyoto
ZIP/Postal code 606-8522
Country Japan
Platform ID GPL17077
Series (1)
GSE77103 Gene expression in peripheral blood mononuclear cells from healthy control and autistic infants

Data table header descriptions
VALUE normalized log2 transformed

Data table
A_23_P11507 4.276706829
A_33_P3319026 4.755854986
A_24_P282108 5.524535682
A_23_P254888 8.991398439
A_23_P426140 5.25704877
A_24_P49533 3.64713799
A_32_P132589 1.635220275
A_33_P3256490 1.68746013
A_23_P303317 2.127863077
A_33_P3295423 5.620936632
A_33_P3334308 4.868622755
A_23_P502627 5.525407139
A_23_P63789 5.604041885
A_33_P3212994 5.333901064
A_33_P3433873 3.838665077
A_23_P64204 5.299884369
A_32_P109522 4.101341964
A_33_P3416420 2.988924904
A_21_P0000778 4.464810292
A_33_P3252989 6.998259474

Total number of rows: 50599

Table truncated, full table size 1255 Kbytes.

Supplementary file Size Download File type/resource
GSM2044364_C4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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