|
| Status |
Public on Apr 04, 2016 |
| Title |
Sample79 Wild type preGM |
| Sample type |
SRA |
| |
|
| Source name |
Wild type mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Bone marrow
strain: C57 BL6
phenotype: 7AAD- Lin- Sca1- cKit+ CD41- FcRII/III- CD105- CD150-
|
| Treatment protocol |
Cells isolated by FACS from mouse bone marrow
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
From single sorted cells, cDNA was generated according to C1™ Single-Cell Auto Prep IFC for mRNA Sequencing (Fluidigm) using the Fluidigm C1 system and following manufacturer protocol.
Illumina sequenecing libraries were constructed in 96-well plates using the Illumina Nextera XT DNA Sample Preparation kit according to a protocol supplied by Fluidigm.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Wild type preGM
|
| Data processing |
Reads were aligned to the murine genome (mm9) using STAR with default parameters.
Non-uniquely mapped reads were discarded. Transcript expression values were quantified using Cufflinks as fragments per kilobase of transcript per million mapped reads (FPKM).
Genome_build: mm9
|
| |
|
| Submission date |
Jan 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Claus Nerlov |
| E-mail(s) |
claus.nerlov@imm.ox.ac.uk
|
| Organization name |
Weatheral Institute of Molecular Medicine
|
| Department |
MRC Molecular Haematology Unit
|
| Lab |
Nerlov Laboratory
|
| Street address |
John Radcliffe Hospital, University of Oxford
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE77029 |
Distinct myeloid progenitor differentiation pathways uncovered through single cell RNA sequencing |
|
| Relations |
| BioSample |
SAMN04431028 |
| SRA |
SRX1538652 |
