|
Status |
Public on Feb 09, 2017 |
Title |
IFC rep2 |
Sample type |
SRA |
|
|
Source name |
maize ovary
|
Organism |
Zea mays |
Characteristics |
developmental state: before pollination tissue: ovary genotype: IFC mutant
|
Treatment protocol |
Under non-stress conditions.
|
Growth protocol |
Zea mays L. (maize) inbred Yu-A474 plants were grown at a research farm of Henan Agricultural University, Henan Province, China (34º53´N, 113 º35´E, 94 m altitude) under non-stress conditions during June-October 2014.
|
Extracted molecule |
total RNA |
Extraction protocol |
The ovaries were isolated by removing the glumes, lemma, and palea and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
FPKM Supplementary_files_format_and_content: FPKM
|
|
|
Submission date |
Jan 12, 2016 |
Last update date |
May 15, 2019 |
Contact name |
hongping li |
E-mail(s) |
lmhlhp021506@163.com
|
Organization name |
Henan Agricultural University
|
Street address |
63
|
City |
zhengzhou |
ZIP/Postal code |
450002 |
Country |
China |
|
|
Platform ID |
GPL17628 |
Series (1) |
GSE76800 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and IFC (incompletely fused carpels) Maize Ovary Transcriptomes |
|
Relations |
BioSample |
SAMN04403354 |
SRA |
SRX1528708 |