|
| Status |
Public on Feb 09, 2017 |
| Title |
WT rep2 |
| Sample type |
SRA |
| |
|
| Source name |
maize ovary
|
| Organism |
Zea mays |
| Characteristics |
developmental state: before pollination
tissue: ovary
genotype: wild type
|
| Treatment protocol |
Under non-stress conditions.
|
| Growth protocol |
Zea mays L. (maize) inbred Yu-A474 plants were grown at a research farm of Henan Agricultural University, Henan Province, China (34º53´N, 113 º35´E, 94 m altitude) under non-stress conditions during June-October 2014.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The ovaries were isolated by removing the glumes, lemma, and palea and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
FPKM
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Jan 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
hongping li |
| E-mail(s) |
lmhlhp021506@163.com
|
| Organization name |
Henan Agricultural University
|
| Street address |
63
|
| City |
zhengzhou |
| ZIP/Postal code |
450002 |
| Country |
China |
| |
|
| Platform ID |
GPL17628 |
| Series (1) |
| GSE76800 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and IFC （incompletely fused carpels） Maize Ovary Transcriptomes |
|
| Relations |
| BioSample |
SAMN04403353 |
| SRA |
SRX1528707 |
