|
| Status |
Public on Oct 29, 2008 |
| Title |
WT3 |
| Sample type |
RNA |
| |
|
| Source name |
wild type, clone 3
|
| Organism |
Homo sapiens |
| Characteristics |
wild type
|
| Treatment protocol |
A549 cells transduced with lentiviral vectors containing specific siRNA sequences against ALDH1A1+ALDH3A1 (Lenti 1+3 cells)
|
| Growth protocol |
A549 cells cultured in RPMI 1640 medium containing 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner G3000
|
| Description |
wild type #3
|
| Data processing |
The data were analyzed with Affymetrix dCHIP using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
|
| Phone |
352 273-8133
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Microarray lab 103
|
| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8045 |
Gene Expression Profiling in A549 Lung Cancer Cell Line Following siRNA Mediated Knock-down of ALDH1A1 and ALDH3A1 |
|
| Relations |
| Reanalyzed by |
GSE119087 |
