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| Status |
Public on Oct 29, 2008 |
| Title |
Gene Expression Profiling in A549 Lung Cancer Cell Line Following siRNA Mediated Knock-down of ALDH1A1 and ALDH3A1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Aldehyde dehydrogenase isozymes ALDH1A1 and ALDH3A1 are highly expressed in non small cell cell lung cancer. Neither the mechanism nor the biological significance for such over expression have been studied.
We used microarrays to analyze changes in A549 lung cancer cell line in which ALDH activity was reduced using lentiviral mediated expression of siRNA against both isozymes (Lenti 1+3)
Keywords: Gene Profiling after ALDH Knock Down
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| Overall design |
A549 lung cancer cell lines were transduced with lentiviral vectors containing specific siRNA sequences against ALDH1A1, ALDH3A1, both vectors (Lenti 1+3 cells), and against the green flourescent protein (GFP) gene (GFP cells, used as control).
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| Contributor(s) |
Moreb JS, Baker HV, Chang L, López C, Ostmark B, Chou W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 06, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
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| Phone |
352 273-8133
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| Organization name |
University of Florida
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| Department |
Molecular Genetics and Microbiology
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| Lab |
Microarray lab 103
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| Street address |
1376 Mowry Road
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA100867 |
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