|
| Status |
Public on Apr 30, 2016 |
| Title |
Reh.shMLXIP |
| Sample type |
RNA |
| |
|
| Source name |
Reh cALL leukemic cell line, shMLXIP
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Reh
cell type: cALL leukemic cell line
shRNA: shMLXIP
|
| Treatment protocol |
cALL leukemic cell lines Nalm6, 697 and Reh were lentivirally infected with inducible MLXIP-specific shRNA and control shRNA. Infected cells were treated for 48h with 1µg/ml doxycycline for specific shRNA induction.
|
| Growth protocol |
Cell lines were grown in plastic cell culture flasks in a humidified incubator at 37°C in 5% CO2 atmosphere in RPMI 1640 media containing 10% heat-inactivated fetal bovine serum, 1% glutamine, 100 µg/mL gentamycin and 200 mg/dl D-glucose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TRIZOL according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (200 ng) was amplified and labeled using Affymetrix GeneChip Whole Transcript Sense Target Labeling Kit.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Human Gene 1.0 ST arrays.
|
| Scan protocol |
GeneChip Scanner 3000 7G System, AGCC software (Affymetrix).
|
| Description |
Reh.shMLXIP
B cell precursor lymphoblastic leukemia cell line with t(5;12) translocation.
|
| Data processing |
RMA normalization using Expression Console software (Build 1.1; Affymetrix).
|
| |
|
| Submission date |
Dec 22, 2015 |
| Last update date |
Apr 30, 2016 |
| Contact name |
Guenther HS Richter |
| E-mail(s) |
guenther.richter@charite.de
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Department of Pediatrics, Division of Oncology and Hematology
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
D-13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE76277 |
MondoA mediates in vivo aggressiveness of common ALL and may serve as a T cell immunotherapy target |
|
