|
Status |
Public on Oct 16, 2015 |
Title |
Healthy control 4 |
Sample type |
RNA |
|
|
Source name |
induced sputum
|
Organism |
Homo sapiens |
Characteristics |
disease state: healthy gender: male age: 24 bmi: 28.7 smoking pack-years: none ID: FAT047
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from induced sputum using the RNeasy Mini Kit (Qiagen, Hilden, Germany);RNA quatitation was performed using the Quanti-iT RiboGreen RNA
|
Label |
biotin
|
Label protocol |
A total of 500 ng of sputum RNA was amplified and labelled with biotin-uridine triphosphate using the Total Prep RNA amplification kit as per manufacturer’s instructions (Life Technologies)
|
|
|
Hybridization protocol |
750 ng cRNA was hybridised to Illumina Sentrix HumanRef-8 V3 Expression BeadChips (Illumina, San Diego, CA, USA) using standard protocols
|
Scan protocol |
Each BeadChip measured the expression of 24,354 genes and was scanned using the Illumina Bead Station and captured using BeadScan 3.5.11 (Illumina)
|
Description |
postmeal
|
Data processing |
Sample and gene profiling results were included in the analysis if the sample was of suitable purity (OD 260/280 ratio between 1.7-2.1) and was successfully amplified (sufficient cRNA generated) and hybridized (95th percentile of fluorescence score>500) and the data passed quality control criteria in GeneSpring GX (correlation coefficients and priciple component analysis plots)
|
|
|
Submission date |
Oct 15, 2015 |
Last update date |
Oct 16, 2015 |
Contact name |
QIAN LI |
E-mail(s) |
c3156151@uon.edu.au
|
Organization name |
University of Newcastle
|
Lab |
HMRI
|
Street address |
University drive
|
City |
Newcastle |
ZIP/Postal code |
2299 |
Country |
Australia |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE74075 |
Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics |
|