NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE74075 Query DataSets for GSE74075
Status Public on Oct 16, 2015
Title Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Consumption of a high fat meal can increase neutrophilic airway inflammation in asthma. This study investigates the molecular mechanisms driving airway neutrophilia following a high fat meal in asthma.
 
Overall design a subset of subjects (asthmatics, n=5; healthy controls, n=3) were examined by microarray. A total of 500 ng of sputum RNA was amplified and labelled with biotin-uridine triphosphate using the Total Prep RNA amplification kit as per manufacturer’s instructions (Life Technologies). 750 ng cRNA was hybridised to Illumina Sentrix HumanRef-8 V3 Expression BeadChips (Illumina, San Diego, CA, USA) using standard protocols. Each BeadChip measured the expression of 24,354 genes and was scanned using the Illumina Bead Station and captured using BeadScan 3.5.11 (Illumina)
 
Contributor(s) Li Q, Baines KJ, Gibson PG, Wood LG
Citation(s) 26751474
Submission date Oct 15, 2015
Last update date Mar 20, 2017
Contact name QIAN LI
E-mail(s) c3156151@uon.edu.au
Organization name University of Newcastle
Lab HMRI
Street address University drive
City Newcastle
ZIP/Postal code 2299
Country Australia
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (16)
GSM1909288 Healthy control 1
GSM1909289 Healthy control 2
GSM1909290 Healthy control 3
Relations
BioProject PRJNA298916

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74075_RAW.tar 3.9 Mb (http)(custom) TAR
GSE74075_non-normalized.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap