|
| Status |
Public on Mar 30, 2016 |
| Title |
CaSR 5 |
| Sample type |
RNA |
| |
|
| Source name |
drug treated tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: neuroblastoma xenograft
treatment: CaSR allosteric activator
|
| Treatment protocol |
Oral gavage, daily
|
| Growth protocol |
Neuroblastoma cells were grown in immunocompromised mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5.5 ug total RNA using WT Plus Reagent kit
|
| |
|
| Hybridization protocol |
5 ug of fragmented and labeled ssDNA was hybridized per array using standard Affymetrix protocols (WT Plus Reagent kit)
|
| Scan protocol |
Arrays were scanned in the Genetitan Platformf for 17 hours at 45ยบ
|
| Data processing |
Data were processed using the limma (v3.24) package in the R (v3.2) environment. Probeset level expression was derived using Robust Multi-array Average normalization. Non-specific filtering of 50% less intense and variable data was applied. Data were summarized and limited to core probe set only.
|
| |
|
| Submission date |
Sep 28, 2015 |
| Last update date |
Mar 30, 2016 |
| Contact name |
Carmen de Torres |
| E-mail(s) |
cdetorres@hsjdbcn.org
|
| Organization name |
Hospital Sant Joan de Deu
|
| Department |
Oncology
|
| Lab |
Developmental Tumor Biology Laboratory
|
| Street address |
Santa Rosa 39
|
| City |
Esplugues |
| State/province |
Barcelona |
| ZIP/Postal code |
08950 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE73506 |
CaSR modulator in neuroblastoma model [mouse] |
| GSE73509 |
CaSR modulator in neuroblastoma model |
|
