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Sample GSM1885097 Query DataSets for GSM1885097
Status Public on Feb 17, 2016
Title RNAseq_IMR-32_mock_noUV_1
Sample type SRA
Source name RNAseq_IMR-32_mock_noUV
Organism Homo sapiens
Characteristics cell line: IMR-32
cell type: neuroblastoma cells
treated with: mock RNAi for 96hrs
sample group: non-stressed
RNAi: mock
Treatment protocol For UV treatment, cells were washed in PBS exposed to 0.5 mJ/cm2 UVC and allowed to recover for 24h before being harvested
RNAi protocol: Cells were treated with mock or nELAVL RNAi for 96 hours before being harvested
Lentiviral infection protocol: Cells were infected with lentivirus 72 hours before being harvested
Growth protocol IMR-32 neuroblastoma cells were grown under standard conditions.
Extracted molecule total RNA
Extraction protocol Trizol extraction
Illumina High-throughput TruSeq RNA Sample preparation: ribominus selection (Invitrogen)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description mock noUV_1
non-stressed mock RNAi
Data processing alignment with Tophat (unambigous mapping; mean inner distance: 20; 20)
inference of exon junctions (Bayesian method)
Genome_build: hg18
Supplementary_files_format_and_content: txt file containing geneID (Entrez) and symbol, raw reads and rpkm values
Submission date Sep 16, 2015
Last update date May 15, 2019
Contact name Claudia Scheckel
Organization name University Hospital Zurich
Department Institute of Neuropathology
Street address Schmelzbergstrasse 12
City Zurich
State/province NY
ZIP/Postal code 8091
Country Switzerland
Platform ID GPL16791
Series (2)
GSE53698 RNAseq in IMR-32 neuroblastoma cells
GSE53699 nELAVL HITS-CLIP and RNA-seq in Alzheimer's Disease patients and IMR-32 neuroblastoma cells
BioSample SAMN04090379
SRA SRX1250589

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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