|
| Status |
Public on Nov 01, 2007 |
| Title |
01L4G: normal control |
| Sample type |
RNA |
| |
|
| Source name |
Ficoll/PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
normal control
|
| Growth protocol |
Whole blood was collected into sodium heparin tubes. PBMCs were separated and placed in Trizol within 4 hrs of blood collection and stored frozen at -80 degree celsius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction using Trizol reagent was performed according to the manufacturer protocol.
|
| Label |
biotin
|
| Label protocol |
Biotio-labeled cDNA was generated using the Nugen Ovation Biotin RNA Amplification and Labeling System
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized to Affymetrix U133plus2.0 Gene Chips
|
| Scan protocol |
A Affymetrix Scanner 3000 was used
|
| Description |
none
|
| Data processing |
Expression values were derived using RMA preprocessing methods. Each probe set was then normalized to the median expression of the control samples.
|
| |
|
| Submission date |
May 07, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
ndate fall |
| E-mail(s) |
ndate.fall@cchmc.org
|
| Phone |
513-636-7663
|
| Organization name |
Cincinnati children's hospital medical center
|
| Department |
rheumatology
|
| Street address |
3333 burnet av
|
| City |
cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7753 |
Gene Expression Profiling in Peripheral Blood in Untreated New Onset Systemic Juvenile Idiopathic Arthritis |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
