|
| Status |
Public on Apr 08, 2010 |
| Title |
Normal luminal YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep2 |
| Sample type |
RNA |
| |
|
| Source name |
normal breast 1089 luminal cell
|
| Organism |
Homo sapiens |
| Characteristics |
stable normal luminal cell with YAP gene knock down
|
| Treatment protocol |
cells were grown to 70-80% before RNA extraction.
|
| Growth protocol |
MBA MB231 was maintained in DMEM with 10% FBS. Normal luminal cell was cultured in the medium for luminal cell.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
gene expression data from stable normal breast luminal cell with YAP gene knock down
|
| Data processing |
RMA robust multi-array analysis using R
|
| |
|
| Submission date |
May 03, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Subham Basu |
| E-mail(s) |
Subham.Basu@cancer.org.uk
|
| URL |
http://science.cancerresearchuk.org/research/loc/london/clincentre_bartslondon/basus/
|
| Organization name |
Cancer Research UK
|
| Department |
Molecular Oncology Unit
|
| Lab |
Cell Survival Signalling Laboratory
|
| Street address |
Charterhouse Square
|
| City |
london |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7700 |
Genes regulated by YAP in normal breast cell line and breast cancer cell lines |
|
| Relations |
| Reanalyzed by |
GSE119087 |
