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Series GSE7700 Query DataSets for GSE7700
Status Public on Apr 08, 2010
Title Genes regulated by YAP in normal breast cell line and breast cancer cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Frequent loss of heterozygosity (LOH) at 11q22-23 in breast cancer strongly suggests that this region contains a tumor suppressor gene, yet to be identified. We and others have shown Yes-associated protein (YAP), which is located at 11q22.2, transactivates the p53 family member p73 upon DNA damage, suggesting a tumor suppressive function for YAP. Our analysis of breast tumor tissues by immunohistochemistry (IHC) showed loss of YAP protein expression in great portion of breast cancers.
We used microarray to look at the targte genes regulated by YAP in normal breast luminal cell and breast cancer cell lines.
Keywords: gene knock down
 
Overall design We generated stable cell lines by introducing control vector(pRS-IRES-GFP/pmig) or YAP shRNA(pRS-IRES-GFP-YAP/pmig-YAP) in a normal breast luminal cell line 1089 luminal and breast cancer cell lines MDA MB231. RAN was extracted and hybridized on Affymetrix microarrays.We looked for new target genes regulated by YAP in normal breast luminal cell and breast cancer cell lines.
 
Contributor(s) Yuan M, Holliday D, Chelala C, Harada T, Gangeswaran R, Ali S, Mein C, Lemoine NR, Jones L, Basu S
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Submission date May 03, 2007
Last update date Mar 25, 2019
Contact name Subham Basu
E-mail(s) Subham.Basu@cancer.org.uk
URL http://science.cancerresearchuk.org/research/loc/london/clincentre_bartslondon/basus/
Organization name Cancer Research UK
Department Molecular Oncology Unit
Lab Cell Survival Signalling Laboratory
Street address Charterhouse Square
City london
ZIP/Postal code EC1M 6BQ
Country United Kingdom
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (10)
GSM187268 MDA MB231 control cell pmig/pRS-IRES-GFP rep1
GSM187269 MDA MB231 control cell pmig/pRS-IRES-GFP rep2
GSM187271 MDA MB231 YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep1
Relations
BioProject PRJNA99467

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Supplementary file Size Download File type/resource
GSE7700_RAW.tar 43.3 Mb (http)(custom) TAR (of CEL)

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