|
| Status |
Public on Aug 01, 2015 |
| Title |
non-fortified lettuce 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Caco-2 colorectal adenocarcinoma cells, non-fortified lettuce, Replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Caco-2 (HTB-37, ATCC)
tissue: Colon
cell type: epithelial
gender/age: Male/72 years adult
|
| Treatment protocol |
Cells were seeded in 6-well culture plates for 24 h. After that, growing medium was replaced by medium containing the extract from iodine-biofortified lettuce/carrot and the extract from non-fortified lettuce/carrot. In all studies, at least 4 wells were examined per treatment. Experiments were repeated 3 times.
|
| Growth protocol |
Cells were cultured in an incubator, under controlled conditions (temperature, 37°C; air, 95%; CO2, 5%), in Eagle's Minimum Essential Medium (Sigma), with fetal bovine serum (FBS) (Gibco) to a final concentration of 20%, according to ATCC procedure.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lettuce and carrot were cultivated and fertilizated with KI as described by Kopeć et al. (2015). The iodine concentration was 0.50 mg/100 g dry mass (d.m.) for biofortified lettuce and 0.12 mg/100 g d.m. for control lettuce (Kopeć et al. 2015). 10 g of fresh lettuce was crushed using a homogenizer (CAT type X 120, USA) and next transferred to the Erlenmaier flask with hot water. Lettuce materials were extracted by shaking (Elpan, water bath shaker type 357, Poland) at 100°C for 2 hours, and next the solution was centrifuged (Centrifuge type MPW-340, Poland). Then, a part of the extracts was used for iodine measurement and other parts were stored at -80 °C for cell culture studies.
|
| Label |
Cy5
|
| Label protocol |
According to the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Colorectal adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Caco-2 (HTB-37, ATCC)
tissue: Colon
cell type: epithelial
gender/age: Male/72 years adult
|
| Treatment protocol |
Cells were seeded in 6-well culture plates for 24 h. After that, growing medium was replaced by medium containing the extract from iodine-biofortified lettuce/carrot and the extract from non-fortified lettuce/carrot. In all studies, at least 4 wells were examined per treatment. Experiments were repeated 3 times.
|
| Growth protocol |
Cells were cultured in an incubator, under controlled conditions (temperature, 37°C; air, 95%; CO2, 5%), in Eagle's Minimum Essential Medium (Sigma), with fetal bovine serum (FBS) (Gibco) to a final concentration of 20%, according to ATCC procedure.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lettuce and carrot were cultivated and fertilizated with KI as described by Kopeć et al. (2015). The iodine concentration was 0.50 mg/100 g dry mass (d.m.) for biofortified lettuce and 0.12 mg/100 g d.m. for control lettuce (Kopeć et al. 2015). 10 g of fresh lettuce was crushed using a homogenizer (CAT type X 120, USA) and next transferred to the Erlenmaier flask with hot water. Lettuce materials were extracted by shaking (Elpan, water bath shaker type 357, Poland) at 100°C for 2 hours, and next the solution was centrifuged (Centrifuge type MPW-340, Poland). Then, a part of the extracts was used for iodine measurement and other parts were stored at -80 °C for cell culture studies.
|
| Label |
Cy3
|
| Label protocol |
According to the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
According to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software.
|
| Description |
Biological replicate 2 of 3. Cells treated with the extract of non-fortified lettuce
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jul 31, 2015 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Magdalena Łój |
| E-mail(s) |
magda.loj@gmail.com
|
| Organization name |
Warsaw University of Life Sciences
|
| Department |
Department of Physiological Sciences
|
| Street address |
Nowoursynowska 166
|
| City |
Warsaw |
| ZIP/Postal code |
02-787 |
| Country |
Poland |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE71605 |
Global changes in gene expression in Caco-2 cell line by treatment with extract from iodine-biofortified carrot and lettuce |
|
