|
| Status |
Public on Aug 27, 2015 |
| Title |
sams-1(RNAi) B |
| Sample type |
RNA |
| |
|
| Source name |
young adult
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
developmental stage: young adult
RNAi: sams-1
choline treatment: no
|
| Treatment protocol |
IPTG concentration was 6mM, choline treated animals were grown on NGM with 30mM choline
|
| Growth protocol |
C. elegans were hatched on to Normal growth media, plus ampicillin, tetracycline and IPTG and harvested with 80% of the animals had reached young adulthood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Tri-regent and cleaned with a Qiaquick RNA column.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using the Affymetrix WT PLUS Reagent Kit from 500ng total RN
|
| |
|
| Hybridization protocol |
3 ug of fragmented and labeled cDNA were hybridized for 16 hrs at 45C on GeneChip C elegans ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
Data was processed using RMA
|
| |
|
| Submission date |
Jul 09, 2015 |
| Last update date |
Aug 27, 2015 |
| Contact name |
Amy K Walker |
| E-mail(s) |
amy.walker@umassmed.edu
|
| Phone |
508-856-3645
|
| Organization name |
UMASS Medical School
|
| Department |
Program in Molecular Medicine
|
| Lab |
Suite 308
|
| Street address |
373 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE70693 |
Comparison of gene expression between sams-1(RNAi) and sams-1(RNAi) animals rescued by choline |
|
