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Sample GSM1812035 Query DataSets for GSM1812035
Status Public on Feb 22, 2016
Title REH-KDM2B
Sample type SRA
 
Source name REH
Organism Homo sapiens
Characteristics cell line: leukemia cell line REH
chip antibody: anti-KDM2B (Millipore, catalog# 09-864)
Growth protocol Human leukemia cell lines were maintained in IMDM supplemented with 10% FBS
Extracted molecule genomic DNA
Extraction protocol Library preapred according to Illumina's instructions
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
 
Data processing FastQC (V 0.10.1) was used to check the data quality. To obatin the peaks, the first 3 bases of the reads were trimmed based on the FastQC results
Samples were aligned to hg19 using bowtie aligner (Version 1.1.1), with default parameters. Only uniquely aligned reads were kept.
Peaks were called used MACS (V1.4.2) with default parameters with p-values < 1e-5.
Genome_build: hg19
Supplementary_files_format_and_content: Peak files were generated by MACS in BED format.
 
Submission date Jul 08, 2015
Last update date May 15, 2019
Contact name Alexandros Tzatsos
E-mail(s) atzatsos@gwu.edu
Phone 2029949104
Organization name George Washington University
Department GW Cancer Center
Lab Cancer Epigenetics Laboratory
Street address 800 22nd St NW SEH 8850
City Washington
State/province DC
ZIP/Postal code 20052
Country USA
 
Platform ID GPL16791
Series (2)
GSE70624 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [ChIP-seq]
GSE70729 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies
Relations
BioSample SAMN03846953
SRA SRX1085065

Supplementary file Size Download File type/resource
GSM1812035_REH_KDM2B_peaks.bed.gz 110.8 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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