|
| Status |
Public on Mar 27, 2007 |
| Title |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=50) |
| Sample type |
RNA |
| |
|
| Source name |
germinated spores, 50 minutes into outgrowth
|
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Characteristics |
Strain Bacillus subtilis 168, 1A700
Time: 50 minutes into outgrowth
|
| Biomaterial provider |
Bart Keijser
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fast RNA pro Blue - Qbiogene
|
| Label |
cy3
|
| Label protocol |
Direct incorporation: Random priming of SSII RT to incorporate cy3-labeled dUTP, followed by NaOH hydrolysis of RNA
|
| |
|
| Hybridization protocol |
Automated slide processor (ASP), GE Healthcare
|
| Scan protocol |
Microarrays were scanned in an Agilent DNA MicroArray Scanner (Agilent Technologies, Palo Alto, CA,USA) at 100 % PMT and 100% laser power
|
| Description |
Data supplied is median cy3 value of 4 normalized technical replicates(duplicate spots on the microarray and duplicate hybridization). Raw data and required information is supplied as supplemental file.
|
| Data processing |
Inconsistent replicate values (duplicate spots on the microarray and duplicate hybridization) were removed if distance to the median was larger than 1 on a log2 scale. Of the remaining replicas, the median value was calculated.
|
| |
|
| Submission date |
Mar 23, 2007 |
| Last update date |
Mar 26, 2007 |
| Contact name |
Bart Keijser |
| Organization name |
TNO
|
| Street address |
Utrechtseweg 48
|
| City |
Zeist |
| ZIP/Postal code |
3704HE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL4415 |
| Series (1) |
| GSE6865 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=0-100) |
|
