|
| Status |
Public on Mar 27, 2007 |
| Title |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=0-100) |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by array
|
| Summary |
Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30 % of the B. subtilis genome.
Keywords: time course, spore outgrowth
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| Overall design |
Time series of outgrowing B subtilis spores (cy-3) with common reference (RNA vegetative cells, TSB OD 0.4, Cy-5). Quality of the spotted oligoarray allows single dye dataanalysis.
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| Contributor(s) |
Keijser B, Ter Beek A, Rauwerda H, Schuren F, Montijn R, van der Spek H, Brul S |
| Citation(s) |
17322312 |
| |
| Submission date |
Jan 25, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Bart Keijser |
| Organization name |
TNO
|
| Street address |
Utrechtseweg 48
|
| City |
Zeist |
| ZIP/Postal code |
3704HE |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL4415 |
Bacillus subtilis 168 spotted oligo array2 |
|
| Samples (14)
|
| GSM177105 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=0) |
| GSM177106 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=5) |
| GSM177107 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=10) |
| GSM177108 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=15) |
| GSM177109 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=20) |
| GSM177110 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=25) |
| GSM177111 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=30) |
| GSM177112 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=40) |
| GSM177113 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=50) |
| GSM177114 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=60) |
| GSM177115 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=80) |
| GSM177116 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=90) |
| GSM177117 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=100) |
| GSM177118 |
Gene expression during Bacillus subtilis spore germination and outgrowth (t=70) |
|
| Relations |
| BioProject |
PRJNA99115 |
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