|
| Status |
Public on Sep 04, 2018 |
| Title |
Patient_27_Varicose |
| Sample type |
RNA |
| |
|
| Source name |
large hollow vein
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 26
stage of the disease, ceap: C2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from homogenized veins using TRIzol according to the manufacturer's protocol. Quality control was performed with electrophoresis of RNA in 1% agarose gel, and its quantity was assessed spectrophotometrically.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using Illumina TotalPrep RNA Amplification kit according to manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Standard Illumina protocol for Direct Hybridization Gene Expression Assay
|
| Scan protocol |
Standard Illumina scanning protocol, iScan System, iScan Control Software
|
| Description |
varicose vein sample (disease condition)
|
| Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio, Gene Expression Module
|
| |
|
| Submission date |
Apr 27, 2015 |
| Last update date |
Sep 04, 2018 |
| Contact name |
Mariya Alexandrovna Smetanina |
| Organization name |
Institute of Chemical Biology and Fundamental Medicine SB RAS
|
| Lab |
Laboratory of Pharmacogenomics
|
| Street address |
8 Lavrentiev Avenue
|
| City |
Novosibirsk |
| ZIP/Postal code |
630090 |
| Country |
Russia |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE68309 |
Genome-wide gene expression profiles of 14 paired (normal and varicose) vein samples from the patients with varicose vein disease. |
| GSE68372 |
Profiles of 20 paired (normal and varicose) vein samples from the patients with varicose vein disease |
|
