|
Status |
Public on Sep 04, 2018 |
Title |
Patient_18_Control |
Sample type |
RNA |
|
|
Source name |
large hollow vein
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 34 stage of the disease, ceap: C2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from homogenized veins using TRIzol according to the manufacturer's protocol. Quality control was performed with electrophoresis of RNA in 1% agarose gel, and its quantity was assessed spectrophotometrically.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using Illumina TotalPrep RNA Amplification kit according to manufacturer’s instructions
|
|
|
Hybridization protocol |
Standard Illumina protocol for Direct Hybridization Gene Expression Assay
|
Scan protocol |
Standard Illumina scanning protocol, iScan System, iScan Control Software
|
Description |
normal vein sample (healthy condition)
|
Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio, Gene Expression Module
|
|
|
Submission date |
Apr 27, 2015 |
Last update date |
Sep 04, 2018 |
Contact name |
Mariya Alexandrovna Smetanina |
Organization name |
Institute of Chemical Biology and Fundamental Medicine SB RAS
|
Lab |
Laboratory of Pharmacogenomics
|
Street address |
8 Lavrentiev Avenue
|
City |
Novosibirsk |
ZIP/Postal code |
630090 |
Country |
Russia |
|
|
Platform ID |
GPL6883 |
Series (2) |
GSE68309 |
Genome-wide gene expression profiles of 14 paired (normal and varicose) vein samples from the patients with varicose vein disease. |
GSE68372 |
Profiles of 20 paired (normal and varicose) vein samples from the patients with varicose vein disease |
|