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Sample GSM1574801 Query DataSets for GSM1574801
Status Public on Sep 01, 2015
Title Nt-ERβ_noE2_2
Sample type SRA
Source name Breast cancer cells
Organism Homo sapiens
Characteristics cell type: MCF-7_WT
cell cultures: (+EtOH -E2)
cell line: MCF-7
treatment: hormone-starved
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cell lines using the standard RNA Extraction method with TRI Reagent (Sigma) method
Indexed libraries were prepared from 1 µg/ea. of purified RNA with TruSeq Stranded total RNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing Basecalls was performed using CASAVA version 1.8
Sequenced reads were mapped to hg19 whole genome using Tophat v.2.0.10
Fragments Per Kilobase of exon per Million reads mapped (FPKM) were calculated using Cuffdiff v2.1.1
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
Submission date Dec 30, 2014
Last update date May 15, 2019
Contact name Antonio Rinaldi
Phone 3282622631
Organization name University of Salerno
Department Faculty of Medicine and Surgery
Lab Laboratory of Molecular Medicine and Genomics
Street address Via Salvatore Allende, 1
City Baronissi
State/province SA
ZIP/Postal code 84081
Country Italy
Platform ID GPL18460
Series (1)
GSE64590 Estrogen Receptor Beta Impacts Hormone-Induced Alternative mRNA Splicing in Breast Cancer Cells
BioSample SAMN03274110
SRA SRX825894

Supplementary file Size Download File type/resource
GSM1574801_Nt-ER_noE2_2_FPKM.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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