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Sample GSM157037 Query DataSets for GSM157037
Status Public on Feb 27, 2007
Title P_aeruginosa_rep1
Sample type RNA
Source name bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated
Organism Homo sapiens
Characteristics bronchial epithelial cells BEAS-2B, P_aeruginosa stimulated, 4h, replicate 1
Extracted molecule total RNA
Extraction protocol RNA was isolated using HighPureTM RNA kit (Roche) as recommended by the manufacturer.
Label biotin
Label protocol Affymetrix one round amplification
Hybridization protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Scan protocol Standard Affymetrix procedures (see GeneChip® Expression Analysis Technical Manual)
Description Human bronchial epithelial cells BEAS-2B were stimulated in vitro with 10E8/ml
UV-inactivated P. aeruginosa or S. aureus or with RSV (MOI 1) for 4 h.
Data processing Expression VALUES were generated using RMA (Robust Multi-Array Average expression measure) using R 2.4.0; Absolute Detection (ABS_CALL) was generated using the function mas5calls of the affy package for R.
Submission date Jan 19, 2007
Last update date Feb 27, 2007
Contact name Joerg Mages
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
Platform ID GPL571
Series (1)
GSE6802 Gene expression analysis of bronchial epithelial cells stimulated by different airway pathogens.

Data table header descriptions
VALUE linear expression value; rma
ABS_CALL MAS 5.0 absolute detection

Data table
1007_s_at 556.42 P
1053_at 613.95 P
117_at 45.96 P
121_at 130.77 M
1255_g_at 11.8 A
1294_at 151.36 P
1316_at 42.47 A
1320_at 46.61 A
1405_i_at 19.81 P
1431_at 12.59 A
1438_at 45.38 A
1487_at 177.9 P
1494_f_at 29.7 A
1598_g_at 566.09 P
160020_at 137.44 P
1729_at 310.46 P
177_at 40.39 P
1773_at 89.82 P
179_at 301.41 A
1861_at 290.77 P

Total number of rows: 22277

Table truncated, full table size 421 Kbytes.

Supplementary file Size Download File type/resource
GSM157037.CEL.gz 2.1 Mb (ftp)(http) CEL

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