|
| Status |
Public on Mar 01, 2007 |
| Title |
40 MIN EGF STIMULATED MCF10A cells |
| Sample type |
RNA |
| |
|
| Source name |
MCF-10A cells spontaneously immortalized normal human mammary epithelial cell line
|
| Organism |
Homo sapiens |
| Characteristics |
MCF10A cells
|
| Treatment protocol |
MCF10A cells were grown in DME:F12 medium supplemented with antibiotics, [10 mg/ml insulin, 0.1 mg/ml cholera toxin, 0.5 mg/ml hydrocortisone, heat-inactivated horse serum (5%); defined as 'serum' in the text] and 10ng/ml EGF. Cells were serum deprived for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction of total RNA was performed using Versagene's RNA isolation kit according to the manufacture's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U!33A2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using laser confocal scanner.
|
| Description |
Gene expression data from 40 MIN EGF STIMULATED MCF10A cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Mar 01, 2007 |
| Contact name |
Tal Shay |
| Organization name |
Weizmann Institute of Science
|
| Department |
Physics of Complex Systems
|
| Street address |
Herzel
|
| City |
Rehovot |
| ZIP/Postal code |
26100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE6784 |
Expression data from MCF10A cells subject to EGF stimulation |
| GSE6786 |
HeLa cells and MCF10A cells subject to EGF stimulation |
|
