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| Status |
Public on Mar 01, 2007 |
| Title |
Expression data from MCF10A cells subject to EGF stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MCF10A cells derived from spontaneously immortalized normal human mammary epithel were subjected to EGF/SERUM stimulation for 0,20,40,60,120,240 and 480 minutes.
We used microarrays to understand the temporal regulation of the cellular EGFR cascade.
Keywords: time course
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| Overall design |
MCF10A cells were grown in DME:F12 medium supplemented with antibiotics, [10 mg/ml insulin, 0.1 mg/ml cholera toxin, 0.5 mg/ml hydrocortisone, heat-inactivated horse serum (5%); defined as 'serum' in the text] and 10ng/ml EGF. Cells were serum deprived for 24 hours. Following stimulation with EGF/SERUM for 0,20,40,60,120,240 and 480 minutes.
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| Contributor(s) |
Ido A, Ami C, Tal S, Eytan D, Yosef Y |
| Citation(s) |
17322878 |
| |
| Submission date |
Jan 18, 2007 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Tal Shay |
| Organization name |
Weizmann Institute of Science
|
| Department |
Physics of Complex Systems
|
| Street address |
Herzel
|
| City |
Rehovot |
| ZIP/Postal code |
26100 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (13)
|
| GSM156774 |
60 MIN EGF STIMULATED MCF10A cells |
| GSM156775 |
120 MIN EGF STIMULATED MCF10A cells |
| GSM156776 |
240 MIN EGF STIMULATED MCF10A cells |
| GSM156777 |
480 MIN EGF STIMULATED MCF10A cells |
| GSM156778 |
20 MIN SERUM STIMULATED MCF10A cells |
| GSM156779 |
40 MIN SERUM STIMULATED MCF10A cells |
| GSM156780 |
60 MIN SERUM STIMULATED MCF10A cells |
| GSM156781 |
120 MIN SERUM STIMULATED MCF10A cells |
| GSM156782 |
240 MIN SERUM STIMULATED MCF10A cells |
| GSM156783 |
480 MIN SERUM STIMULATED MCF10A cells |
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| This SubSeries is part of SuperSeries: |
| GSE6786 |
HeLa cells and MCF10A cells subject to EGF stimulation |
|
| Relations |
| BioProject |
PRJNA104123 |
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