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Sample GSM1547793 Query DataSets for GSM1547793
Status Public on Nov 30, 2015
Title ptREX-NIK3
Sample type RNA
Source name MCF7 cell line
Organism Homo sapiens
Characteristics cell line: MCF7
sample type: NIK overexpressed
Treatment protocol Only the transfection
Growth protocol MCF7 cells were transiently transfected with a shRNA-NIK using lipofectamine 2000 (invitrogen) . A shRNA directed against luciferase (siRNA-Luc) was employed as a control. In addition, to overexpress NIK, MCF7 cells were transiently transfected with an expression vector (pt-REX-NIK), which contains the complete ORF of NIK. . The cells were analyzed for NIK expression 48 hours after transfection
Extracted molecule total RNA
Extraction protocol RNA Extraction with Trizol
Label non
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Gene expression data of MCF7ptREX-NIK3
Data processing Raw CEL data were normalized using the Robust Multi-array Average (RMA) method, as implemented in the oligo package . The Limma R package was used for differential expression analysis . We identified genes with a >2-fold change and a crude p<0.05.
Submission date Nov 17, 2014
Last update date Nov 30, 2015
Contact name Vilma Araceli Maldonado
Phone 5553501900
Organization name INMEGEN
Department Investigacion
Lab Number 3
Street address Periferico 4809
City México
State/province Distrito Federal
ZIP/Postal code 14610
Country Mexico
Platform ID GPL10739
Series (1)
GSE63382 Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells

Data table header descriptions

Data table
7892501 6.30225
7892502 6.20199
7892503 4.89223
7892504 10.31669
7892505 3.9705
7892506 6.72104
7892507 7.59387
7892508 9.2615
7892509 12.715
7892510 5.79682
7892511 5.3631
7892512 8.8738
7892513 3.34232
7892514 12.53428
7892515 10.55374
7892516 5.97634
7892517 7.28911
7892518 5.30578
7892519 6.78959
7892520 10.323

Total number of rows: 65528

Table truncated, full table size 1028 Kbytes.

Supplementary file Size Download File type/resource
GSM1547793_KVS009.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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