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Sample GSM152351 Query DataSets for GSM152351
Status Public on Mar 10, 2007
Title 484T
Sample type RNA
Source name Mammary gland from patient with human breast cancer
Organism Homo sapiens
Characteristics Human breast cancer
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label Biotin
Label protocol cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description Age at diagnosis: 53, Histology: IDC, Tumor stage: 1, Tumor grade: 3
Data processing The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
Submission date Dec 21, 2006
Last update date Mar 09, 2007
Contact name Andreas Klein
Phone +49 30 450 528087
Fax +49 30 450 52945
Organization name Charité (CCO)
Department Institute of Biochemistry
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platform ID GPL96
Series (2)
GSE6596 Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes
GSE6772 Comparison of gene expression data from human and mouse breast cancers

Data table header descriptions
VALUE The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
ABS_CALL The call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
1007_s_at 336 P 0.002228
1053_at 159.2 P 0.017001
117_at 59.6 P 0.048995
121_at 227.8 P 0.014937
1255_g_at 24.9 P 0.039365
1294_at 75.6 P 0.024711
1316_at 60.3 P 0.014937
1320_at 29 A 0.397994
1405_i_at 160 P 0.001165
1431_at 17.7 A 0.127645
1438_at 23.4 A 0.52062
1487_at 149.5 M 0.05447
1494_f_at 110.7 P 0.009985
1598_g_at 305.2 P 0.014937
160020_at 146.4 P 0.031336
1729_at 88 P 0.003585
177_at 29.9 A 0.175989
1773_at 63.2 A 0.189687
179_at 129.3 A 0.081337
1861_at 40 A 0.267463

Total number of rows: 22283

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM152351.CEL.gz 3.7 Mb (ftp)(http) CEL

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