|
| Status |
Public on Jan 10, 2015 |
| Title |
T-DNA insertion line atpip1;2-1, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
3 weeks old leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: atpip1_2-1
tissue: leaf material
|
| Growth protocol |
plants were grown in the greenhouse 3 weeks. 12-h photoperiod (24°C day/20°C night), 60% relative humidity; a photon flux density at plant level of about 70 to 80 µmol m-2s-1 and an ambient CO2 concentration of 370 µmol mol-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 5 individual plants for each microarray (15 plants from each line in total) using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA)
|
| Label |
Biotin
|
| Label protocol |
GeneChip WT Terminal Labelling Kit (Affymetrix, USA) and WT Expression Kit (Ambion, USA)
|
| |
|
| Hybridization protocol |
According to the Hybridization, Wash and Stain Kit manual (Affymetrix, USA). The resulting target was hybridized to the GeneChip AraGene-1.0 ST arrays (Affymetrix, USA) for 17 hrs at 45 °C (3 plants from each line)
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
atpip1_2-1 #2
|
| Data processing |
Images were processed using Partek’s Genomic Suite’s workflow designed for Exon-Arrays. Chp-files were generated applying quantile normalization and the RMA summarization algorithm (Irizarry et al. 2003).
|
| |
|
| Submission date |
Oct 08, 2014 |
| Last update date |
Jan 10, 2015 |
| Contact name |
Anastassia Boudichevskaia |
| E-mail(s) |
boudichevskaia@bio.tu-darmstadt.de
|
| Phone |
+496151163505
|
| Organization name |
TU Darmstadt
|
| Department |
FB Biologie
|
| Lab |
Applied Plant Sciences
|
| Street address |
Schnittspahnstrasse 10
|
| City |
Darmstadt |
| State/province |
Hessen |
| ZIP/Postal code |
64287 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17416 |
| Series (1) |
| GSE62167 |
Expression data from the T-DNA insertion line atpip1;2-1 |
|
