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Sample GSM1519592 Query DataSets for GSM1519592
Status Public on Oct 07, 2014
Title CD161-_2
Sample type RNA
Source name CD4 T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD4+ T cells
Treatment protocol CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in CD161- CD4+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62095 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.916155623 0.3083
ILMN_2055271 6.055964157 0.16733
ILMN_1736007 5.831413943 0.41502
ILMN_2383229 5.703219787 0.62582
ILMN_1806310 5.708366831 0.61792
ILMN_1779670 6.172281011 0.07378
ILMN_2321282 5.776112355 0.50725
ILMN_1671474 5.690271261 0.64032
ILMN_1772582 5.857131441 0.38472
ILMN_1735698 6.199951788 0.06456
ILMN_1653355 6.57300327 0.00395
ILMN_1717783 5.368078094 0.8946
ILMN_1705025 6.164811617 0.07905
ILMN_1814316 5.437910926 0.85639
ILMN_2359168 5.907230678 0.32543
ILMN_1731507 5.763804532 0.5336
ILMN_1787689 5.674578047 0.66008
ILMN_1745607 5.397055791 0.87879
ILMN_2136495 5.987752794 0.22925
ILMN_1668111 5.810025932 0.45191

Total number of rows: 48802

Table truncated, full table size 1501 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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