|
| Status |
Public on Oct 07, 2014 |
| Title |
CD161-_2 |
| Sample type |
RNA |
| |
|
| Source name |
CD4 T cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral Blood cells
cell type: CD4+ T cells
|
| Treatment protocol |
CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
|
| Growth protocol |
Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using a Qiagen Rneasy kit
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
|
| |
|
| Hybridization protocol |
A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
|
| Scan protocol |
Chips were scanned with Illumina BeadArray Reader accordingly
|
| Description |
Gene expression in CD161- CD4+ T cells
|
| Data processing |
GenomeStudio (Illumina Inc) was used for data extraction accordingly
|
| |
|
| Submission date |
Oct 06, 2014 |
| Last update date |
Oct 07, 2014 |
| Contact name |
Joannah R Fergusson |
| Organization name |
University of Oxford
|
| Department |
Peter Medawar Building for Pathogen Research
|
| Street address |
South Parks Road
|
| City |
Oxford |
| State/province |
select a state |
| ZIP/Postal code |
OX1 3SY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE62095 |
CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4) |
| GSE62099 |
CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages |
|
