|
| Status |
Public on Oct 08, 2015 |
| Title |
patient 32 with ALF - whole liver tissue miRNA - miRNA-ALF-32-1 |
| Sample type |
RNA |
| |
|
| Source name |
specimen obtained from explanted liver of patient 32
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole liver
phenotype: from OLT of HBV-associated acute liver failure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including microRNA,was extracted from frozen liver specimens using the miRNeasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
500ng of total RNA, including microRNA, was poly(A) tailed and then directly ligated to a fluorescent dendrimer (a branched single and double stranded DNA molecule conjugated to biotin) using the FlashTag Biotin HSR RNA Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed on Affymetrix GeneChip miRNA 2.0 arrays using standard protocols available at www.affymetrix.com.
|
| Scan protocol |
Standard Affymetrix protocols were also used for staining, washing, and scanning of the arrays (available at www.affymetrix.com).
|
| Data processing |
Affymetrix probeset summaries were computed using the RMA algorithm. Data were then imported to BRB-ArrayTools and only the 2226 human miRNAs present in the chip were filtered and used for the subsequent analyses. Median normalization, group comparisons and multivariate analyses were made using BRB-ArrayTools and R packages.
|
| |
|
| Submission date |
Oct 03, 2014 |
| Last update date |
Oct 08, 2015 |
| Contact name |
Giacomo Diaz |
| E-mail(s) |
gdiaz@unica.it
|
| Organization name |
University of Cagliari
|
| Department |
Dept Biomedical Sciences
|
| Street address |
Via Porcell 4
|
| City |
Cagliari |
| ZIP/Postal code |
09124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE62030 |
Integrated ordination of miRNA and mRNA expression profiles [miRNA] |
| GSE62037 |
Integrated ordination of miRNA and mRNA expression profiles |
|
