|
| Status |
Public on Oct 02, 2015 |
| Title |
Bt_B22M_18 |
| Sample type |
RNA |
| |
|
| Source name |
Bull_treated TBA(200 mg)+BE2(40 mg)
|
| Organism |
Bos taurus |
| Characteristics |
tissue: Muscle
breed: Charolais
gender: male
age: 10-14 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from liver and muscle tissues' aliquots was isolated using Trizol reagent according to manufacturer's instruction and purified according to Qiagen RNeasy Mini Kit protocol for tissue samples.
|
| Label |
Cy3
|
| Label protocol |
Amplification and labelling was done according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Tissue aliquots from the muscle biceps brachii were aseptically collected after slaughtering the animal, submerged in RNAlater solution and stored at –80 °C until extraction
18_Bt_B22M_565_2
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using R2.15.0 statistical software. Data from each matrix were analyzed and filtered separately to avoid any bias.
Normalized with quantile method; 'blank' (no value) corresponds to probes not considered for the normalization step; null corresponds to missing data from analysis and/or probes (genes) that didn't pass the filtering step.
|
| |
|
| Submission date |
Oct 02, 2014 |
| Last update date |
Oct 02, 2015 |
| Contact name |
Ramy Elgendy |
| E-mail(s) |
ramy.elgendy@astrazeneca.com
|
| Organization name |
AstraZeneca
|
| Street address |
Pepparedsleden 1
|
| City |
Mölndal |
| State/province |
Göteborg |
| ZIP/Postal code |
43153 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE62002 |
The transcriptome of muscle and liver is responding differently to a combined trenbolone acetate and estradiol implant in cattle |
|
