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Sample GSM1516169 Query DataSets for GSM1516169
Status Public on Dec 17, 2015
Title MDM-24h-dex100nM-rep3
Sample type RNA
Source name monocyte derived macrophage
Organism Homo sapiens
Characteristics source: fully anonymized volunteers
cell type: monocyte derived macrophage
time: 24h
treatment: dex 100nM
Treatment protocol Samples were treated with 100nM dexamethasone (Sigma) for the indicated duration
Growth protocol CD14+ monocytes were extracted from whole blood donated by healthy volunteers using MACS separation (Miltenyi). They were differentiated in culture for 1 week in the presence of recombinant human CSF1 (10000U/ml),penicillin/streptomycin, Glutamax and 10% fetal calf serum then replated in fresh medium, also supplemented as above, for the time series experiment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNeasy kit (Qiagen) and quality assessed using an 2100 Bioanalyzer (Agilent )
Label biotin
Label protocol Standard affymetrix protocols, Performed by Edinburgh Genomics.
Hybridization protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Scan protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Data processing Raw data was processed using R/Bioconductor: arrayQualityMetrics for quality control and the rma algorithm in the affy package to generate expression values.
Submission date Sep 29, 2014
Last update date Dec 17, 2015
Contact name Alasdair W Jubb
Organization name University of Edinburgh
Department Roslin Institute
Lab Hume lab / Bickmore lab (MRC HGU)
Street address The Roslin Institute
City Easter Bush
State/province Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
Platform ID GPL13158
Series (2)
GSE61880 Expression response of human monocyte derived macrophages to dexamethasone over a 24h time series
GSE61881 Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers

Data table header descriptions
VALUE unlogged rma signal

Data table
1007_PM_s_at 37.19291244
1053_PM_at 102.3226255
117_PM_at 28.86171618
121_PM_at 109.1875066
1255_PM_g_at 5.907893459
1294_PM_at 101.3758505
1316_PM_at 26.93265346
1320_PM_at 6.591650874
1405_PM_i_at 18.17371061
1431_PM_at 8.446026721
1438_PM_at 6.239262695
1487_PM_at 71.36329227
1494_PM_f_at 10.7338114
1552256_PM_a_at 352.9897896
1552257_PM_a_at 158.9400168
1552258_PM_at 6.212943191
1552261_PM_at 6.879969247
1552263_PM_at 300.9194766
1552264_PM_a_at 191.5308212
1552266_PM_at 7.249433871

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.

Supplementary file Size Download File type/resource
GSM1516169_H3_24.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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