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| Status |
Public on Dec 17, 2015 |
| Title |
Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid receptor (GR) was detected at candidate enhancers in the vicinity of induced genes in both species and this was strongly associated with canonical GR binding motifs. However, the sets of inducible genes, the candidate enhancers, and the GR motifs within them, were highly-divergent between the two species.
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
26663721, 29241532 |
| |
| Submission date |
Sep 29, 2014 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Alasdair W Jubb |
| E-mail(s) |
alasdair.jubb@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Roslin Institute
|
| Lab |
Hume lab / Bickmore lab (MRC HGU)
|
| Street address |
The Roslin Institute
|
| City |
Easter Bush |
| State/province |
Midlothian |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
| |
|
| Platforms (4)
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| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (52)
|
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| This SuperSeries is composed of the following SubSeries:
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| GSE61877 |
Genome wide binding of glucocorticoid receptor in dexamethasone treated mouse bone marrow derived macrophages |
| GSE61878 |
Genome wide binding of glucocorticoid receptor in dexamethasone treated human monocyte derived macrophages |
| GSE61879 |
Expression data from a 24h time series of dexamethasone treatment for mouse bone marrow derived macrophages |
| GSE61880 |
Expression response of human monocyte derived macrophages to dexamethasone over a 24h time series |
|
| Relations |
| BioProject |
PRJNA262584 |
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