|
| Status |
Public on May 19, 2015 |
| Title |
OHT-3 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: E14.5
tissue: liver
|
| Treatment protocol |
The cells were treated with 100nM OHT or EtOH (control) for 24h.
|
| Growth protocol |
Lineage negative cells were isolated from E14.5 mouse fetal livers and cultured ex vivo.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Life Technologies' PureLink RNA mini kit according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
cRNA was prepared with Illumina® TotalPrep RNA kit according to the manufacturer's protocol Illumina Total Prep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with limma and beadarray in R
|
| |
|
| Submission date |
Sep 22, 2014 |
| Last update date |
May 19, 2015 |
| Contact name |
Diana HP Low |
| E-mail(s) |
dlow@imcb.a-star.edu.sg
|
| Organization name |
Institute of Molecular and Cell Biology
|
| Lab |
Chromatin, Epigenetics & Differentiation
|
| Street address |
#03-06, 61 Biopolis Drive, Proteos
|
| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE61637 |
Regulation of the core pre-mRNA splicing machinery by MYC and PRMT5 is essential to sustain lymphomagenesis [fetal liver] |
| GSE61638 |
Regulation of the core pre-mRNA splicing machinery by MYC and PRMT5 is essential to sustain lymphomagenesis |
|
