|
| Status |
Public on Jun 17, 2015 |
| Title |
HeLa-CDK9-inhibitor-T2-3 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa-CDK9-inhibitor-T2-3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
treatment: CDK9 inhibitor
time (hours): 2
|
| Treatment protocol |
HeLa cells were treated with DMSO or i-CDK9 for 2, 8 or 16 hours
|
| Growth protocol |
HeLa cells were cultured in DMEM supplemented with 10% FBS. Cells were maintained at 37 °C under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNAeasy Plus kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
CDK9 inhibitor treatment at 2hr
|
| Data processing |
The data were analyzed with Bioconductor affy package (http://bioconductor.org) with RMA method and HGU133Plus2_Hs_ENTREZG custom CDF v14.1 for target gene definition (Dai et al., 2005)
|
| |
|
| Submission date |
Sep 11, 2014 |
| Last update date |
Jun 17, 2015 |
| Contact name |
Zhenhai Gao |
| E-mail(s) |
zgao@incyte.com
|
| Organization name |
Novartis Institute for Biomedical Research
|
| Street address |
4560 Horton street
|
| City |
emeryville |
| State/province |
California |
| ZIP/Postal code |
94608 |
| Country |
USA |
| |
|
| Platform ID |
GPL18756 |
| Series (2) |
| GSE60952 |
Expression profiling of HeLa cell line treated with CDK9 inhibitor |
| GSE60954 |
Expression profiling and Pol II occupancy of HeLa cell line treated with CDK9 inhibitor |
|
