|
| Status |
Public on Sep 30, 2014 |
| Title |
EPCdEC of the first donor at passage 12 |
| Sample type |
RNA |
| |
|
| Source name |
Human adult bone marrow mononuclear cell-derived endothelial progenitor outgrowth cells (EPOCs): lot B112054, BioChain Institute, Inc., Hayward, CA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: vascular endothelical cells
|
| Growth protocol |
Vascular endothelia cells were cultured on 0.1% gelatin-coated plates using EGM®-2 BulletKit (Lonza Group Ltd. Basel, Switzerland) . Cells were subcultured at split ratios of 1:2 ~ 1:3 twice a week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated by using TRIzoL® Reagent (Life Technologies, Inc., Grand Island, NY).
|
| Label |
biotin
|
| Label protocol |
Samples were labeled according to the Affymetrix standard protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized according to the Affymetrix standard protocol.
|
| Scan protocol |
An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
|
| Data processing |
Normalized expression signal data was generated at the exon level using Affymetrix's Expression Console by RMA.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Kumiko Saeki |
| Organization name |
National Center for Global Health and Medicine
|
| Department |
Department of disease control
|
| Street address |
1-21-1 Toyama
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE61000 |
Effects of differences in donors and passage numbers of endothelial progenitor cell (EPC)-derived vascular endothelial cells (VECs) on the phenotype determination of VECs (type-I versus type-II). |
|
| Relations |
| Alternative to |
GSM1495430 (gene-level analysis) |
