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Sample GSM1495434 Query DataSets for GSM1495434
Status Public on Sep 30, 2014
Title EPCdEC of the first donor at passage 12
Sample type RNA
Source name Human adult bone marrow mononuclear cell-derived endothelial progenitor outgrowth cells (EPOCs): lot B112054, BioChain Institute, Inc., Hayward, CA
Organism Homo sapiens
Characteristics cell type: vascular endothelical cells
Growth protocol Vascular endothelia cells were cultured on 0.1% gelatin-coated plates using EGM®-2 BulletKit (Lonza Group Ltd. Basel, Switzerland) . Cells were subcultured at split ratios of 1:2 ~ 1:3 twice a week.
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated by using TRIzoL® Reagent (Life Technologies, Inc., Grand Island, NY).
Label biotin
Label protocol Samples were labeled according to the Affymetrix standard protocol.
Hybridization protocol Samples were hybridized according to the Affymetrix standard protocol.
Scan protocol An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
Data processing Normalized expression signal data was generated at the exon level using Affymetrix's Expression Console by RMA.
Submission date Sep 02, 2014
Last update date Sep 30, 2014
Contact name Kumiko Saeki
Organization name National Center for Global Health and Medicine
Department Department of disease control
Street address 1-21-1 Toyama
City Shinjuku-ku
State/province Tokyo
ZIP/Postal code 162-8655
Country Japan
Platform ID GPL10739
Series (1)
GSE61000 Effects of differences in donors and passage numbers of endothelial progenitor cell (EPC)-derived vascular endothelial cells (VECs) on the phenotype determination of VECs (type-I versus type-II).
Alternative to GSM1495430 (gene-level analysis)

Data table header descriptions
VALUE RMA signal intensity

Data table
7892501 64.93714266
7892502 17.46237641
7892503 7.710952068
7892504 638.7050291
7892505 24.44749936
7892506 18.4865102
7892507 14.88675081
7892508 17.46675861
7892509 5214.958295
7892510 21.07709306
7892511 8.379522294
7892512 118.9253944
7892513 19.8079133
7892514 1984.073836
7892515 1003.549456
7892516 66.14292769
7892517 23.13578267
7892518 7.658383998
7892519 49.67131376
7892520 744.912933

Total number of rows: 257430

Table truncated, full table size 4995 Kbytes.

Supplementary file Size Download File type/resource
GSM1495434_PF090804_01.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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